Isoschizomers: BsmBI, BstGZ53I, Esp16I, Esp23I.Thermo Scientific FastDigest Esp3I is one of an advanced line o
Thermo Scientific Esp3I (BsmBI) restriction enzyme recognizes CGTCTC (1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer. SalI has a High Fidelity version SalI-HF ® (). A restriction endonuclease that recognizes the sequence G^TCGA_C. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. To comprehensively map TSS of the S. meliloti 1021 transcriptome, RNA populations derived from 16 different growth and stress conditions were pooled and used for cDNA library preparation (Methods). For enzymes that recognize non-palindromic sequences, the complementary sequence of each strand is listed. As an example, BsmBI has a recognition site of CGTCTC(N1/N5), where the CGTCTC represents the recognition/binding site, and the N1/N5 indicates the cut site is one base downstream on the top strand, and five bases downstream on the bottom strand. Note: Also available as a FastDigest enzyme for rapid DNA digestion. These conditions included exponential and stationary phase growth in three different media, temperature and pH shifts, oxidative and high salt … Type IIS Restriction Enzymes Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence. 5'CGTCTCN13'3'GCAGAGN55'Thermo Scientific FastDigest Esp3I (IIs class) restriction enzyme recognizes CGTCTC(1/5)^ site and cuts best at 37C in 515 minutes using universal FastDigest Buffer. Restriction Enzymes Thermo Scientific™ Eam1104I (EarI) (10 U/µL) The Eam1104I (EarI) restriction enzyme recognizes CTCTTC(1/4)^ sites and cuts best at … Thus, a single Type IIS restriction enzyme can be used to generate DNA fragments with unique overhangs. All restriction endonuclease recognition specificities available from New England Biolabs are listed below. High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. Global mapping of transcription start sites. This table allows you to sort our enzymes by feature for easy comparison. They are useful for many applications, including Golden Gate Assembly. NEB currently offers over 45 Type IIS restriction enzymes. For example, CCTC(7/6) and (6/7)GAGG both represent an MnlI site.
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