This system allows for double and multiple digestions with any combination of enzymes. Thermo Scientific FastDigest BamHI restriction enzyme recognizes G^GATCC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
Brand: Thermo Scientific™ FD0594 The 10X FastDigest Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. – 0.2 µg of PCR product in 30 min. FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Buffer.
Required but not provided: DTT. n io t ic r Ret s t s e Dig t s a F Enzymes Inactivation The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI). Therefore, enzyme
Shop now at Fisher Scientific for all of your scientific needs. The blue dye of the FastDigest Green Buffer migrates with 3-5 kb DNA fragments in a 1% agarose gel and has an excitation peak at 424 nm. No sequential digestions or buffer changes are needed. FastDigest AflII (BspTI) (150 rxns) from Thermo Fisher Scientific Description The FastDigest AflII (BspTI) restriction enzyme recognizes C^TTAAG sites and cuts best at 37°C in 5-15 minutes using universal Yellow Tango Buffer (Isoschizomers: BfrI, Bst98I, BstAFI, MspCI, Vha464I) The 10X FastDigest Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. 100% activity in a single buffer.
– 1 µg of plasmid DNA in 20 min. Thermo Scientific™ FastDigest XhoI XhoI; 400uL Thermo Scientific™ FastDigest XhoI Xa - Zz Restriction ... Two versions of the universal FastDigest buffer are supplied with each enzyme: 10X FastDigest Buffer and 10X FastDigest Green Buffer.
100% activity in FastDigest and FastDigest Green Buffer. • Incubation at 37°C. The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps.
DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Buffer.
AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, New Lab Start-Up Program; Controlled Environments; Supplier Diversity Program ; Globally Harmonized System; Sustainability Program; Global Solutions; Compounding Pharmacy Product Guide; Production Solutions; Literature & Subscriptions. They even invented a new activity unit for these enzymes (Fast Digest Unit: 1 FDU cuts 1 µg control DNA in 5 min in FastDigest buffer).
... DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. The 10X FastDigest Green Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. One buffer for 176 enzymes—it’s that easy Thermo Scientific ™ FastDigest Restriction Enzymes support complete and fast digestions.